PUBLICATIONS
For the complete list of publications, including works published before 2019, please refer to Google Scholar. Our open access papers can also be found on our Zenodo community.
2026
Ribezzi D, Catala P, Groessbacher G, Bernal P, Nijssen O, Florczak S, Nijenhuis W, Crusellas-Villorbina N, Nijhoff B, Delrot P, Malda J, Hierholzer A, Fussenegger M, Kapitein L, Levato R. Spatial Control of Light-Responsive Proteins and Optogenetics Within Hydrogels via Volumetric Bioprinting. Advanced Materials. doi: 10.1002/adma.74563
Sumbelli Y, Gines-Rodriguez N, Paffen L, Nikroo A, Levato R, van Hest J, Malda J. Patterning of Artificial Cell Populations Via Laser Induced Forward Transfer for Controlled and Localized Cargo Uptake and Release. Small. doi: 10.1002/smll.76083
Prados-Martin L, Tran H, Mota C, Jang J, Zenobi-Wong M, Daly A, Jungst T, van Vlierberghe S, Burdick J, Shrike Zhang Y, Woodfield T, Levato R, Lim K. Advances in light-based 3D printing. Biofabrication. doi: 10.1088/1758-5090/ae7208
Blanco A, Rubio-Pego I, Bao-Varela C, Bouza R, Bernal P, Levato R, Malda J, Kon E, Crecente-Campo J, Alonso M. Polymer Coating Adhesion Drives Functional and Biological Outcomes in Meniscus Implants. ACS Applied Materials & Interfaces. doi: 10.1021/acsami.6c10220
Ksiezarczyk M, IJsseldijk L, van Weeren R, Levato R, Malda J. Age-dependent development and microarchitecture of the osteochondral unit of the humeral head in harbour porpoises (Phocoena phocoena). Scientific Reports. doi: 10.1038/s41598-026-39726-7
Vasilopoulou A, Gines-Rodriguez N, Mansouri A, Spauwen L, Loste-Berdot M, Iazzolino A, Nicodeme M, Ilsievic L, Korpershoek J, Levato R, de Ruijter M, Malda J, Converging Laser-Induced Forward Transfer and Melt ElectroWriting for biofabrication of reinforced cartilage constructs. Biofabrication. doi: 10.1088/1758-5090/ae3c43
Pol F, Longoni A, Levato R, Gawlitta D, Man K. Extracellular vesicles in osteoimmunomodulation: orchestrating immune-driven bone regeneration. International Journal of Biological Macromolecules. doi: 10.1016/j.ijbiomac.2025.149614
2025
Dudaryeva O, Buchholz M, Groessbacher G, Amaral S, Florczak S, Bonhomme C, Rojo-Ferrer A, Tibbitt M, Levato R. Multi-scale Engineered Vasculature and Hierarchical Porosity via Volumetric Bioprinting-Guided Photopolymerization-Induced Phase Separation. Advanced Materials. doi: 10.1002/adma.202521171
Bosmans C, Gines-Rodriguez N, Gutierrez-Hernandez U, Fernandez Rivas D, Karperien M, Malda J, Moreira-Teixeira L, Levato R, Leijten J. A quantitative printability framework for programmable assembly of pre-vascular patterns via laser-induced forward transfer. Advanced Healthcare Materials. doi: 10.1002/adhm.202503665
Florczak S, Groessbacher G, Ribezzi D, Longoni A, Gueye M, Grandidier E, Malda J, Levato R. Adaptive and context-aware volumetric printing. Nature. doi: 10.1038/s41586-025-09436-7
Lee S, Jang J, Levato R. Advances in light-based process for cell-based 3D bioprinting applications. Materials Today Bio. doi: 10.1016/j.mtbio.2025.102024
Aristin S, Cutilli A, Cambiaso E, Rockx-Brouwer D, Frederiks C, Falandt M, Levato R, Kranenburg O, Lindemans C, Coffer P, Peperzak V, Mocholi E, Cuenca M. Impact of 3D cell culture hydrogels derived from basement membrane extracts or nanofibrillar cellulose on CAR-T cell activation. iScience. doi: 10.1016/j.isci.2025.113234
Iudin D, Gerridzen L, Bernal P, Schuurmans C, Neumann M, Nguyen L, van Steenbergen M, Hak J, Li W, Casadidio C, van Genderen A, Masereeuw R, Levato R, Zhang Y, van Ravensteijn B, Vermonden T. In-Depth Investigation of Electrostatic Interaction-Based Hydrogel Shrinking for Volumetric Printing and Tissue Engineering Applications. Biomacromolecules. doi: 10.1021/acs.biomac.5c00117
Palmieri F, Gueye M, Vicario derl Rio L, Bunschuh S, Chopra P, Mihaila M, Vermonden T, Levato R, Boons G. Photo-triggered Hyaluronic Acid Hydrogel for 3D Culture and Cell Recovery. ChemBioChem. doi: 10.1002/cbic.202500310
Lopetegui-Aizarna U, Groessbacher G, Herrero-Ruiz A, Tejo-Otero A, Henriksen-Lacey M, Levato R, Jimenez de Aberasturi D. Hybrid Plasmonic Bioresins and dECM-Based Materials for Volumetric Bioprinting of Vascular-Inspired Architectures. ACS Applied Materials and Interfaces. doi: 10.1021/acsami.5c03880
Elkadi O, Abinzano F, Nippolainen E, Gonzalez O, Toyras J, Levato R, Malda J, Afara I. Non-Destructive Assessment of Tissue Engineered Cartilage Maturity Using Visible and Near Infrared Spectroscopy Combined with Machine Learning. Biosensors and Bioelectronics. doi: 10.1016/j.bios.2025.117587
Buchholz M, Bernal P, Bessler N, Bonhomme C, Levato R, Rios A. Development of a bioreactor and volumetric bioprinting protocol to enable perfused culture of biofabricated human epithelial mammary ducts and endothelial constructs. Biofabrication. doi: 10.1088/1758-5090/add20f
Di Marco G, Falandt M, Neumann M, Viola M, Sampon T, Valverde M, van Genderen A, Mihaila S, van Nostrum C, van Ravensteijn B, Levato R, Masereeuw R, Vermonden T. Tunable Thermoshrinkable Hydrogels for 4D Fabrication of Cell Seeded Channels. Advanced Functional Materials. doi: 10.1002/adfm.202502042
Ribezzi D, Catala P, Pignatelli C, Citro A, Levato R. Bioprinting and synthetic biology approaches to engineer functional endocrine pancreatic constructs. Trends in Biotechnology. doi: 10.1016/j.tibtech.2025.03.005
Bernal P, Florczak S, Inacker S, Kuang X, Madrid-Wolff J, Regehly M, Hecht S, Zhang YS, Moser C, Levato R. The road ahead in materials and technologies for volumetric 3D printing. Nature Reviews Materials. doi: 10.1038/s41578-025-00785-3
Ribezzi D, Zegwaart JP, Van Gansbeke T, Tejo-Otero A, Florczak S, Aerts J, Delrot P, Hierholzer A, Fussenegger M, Malda J, Olijve J, Levato R. Multi-material Volumetric Bioprinting and Plug-and-play Suspension Bath Biofabrication via Bioresin Molecular Weight Tuning and via Multiwavelength Alignment Optics. Advanced Materials. doi: 10.1002/adma.202409355
Dudaryeva O, Cousin L, Krajnovic L, Grobli G, Sapkota V, Ritter L, Deshmukh D, Cui Y, Style R, Levato R, Labouesse C, Tibbitt M. Tunable Bicontinuous Macroporous Cell Culture Scaffolds via Kinetically Controlled Phase Separation. Advanced Materials. doi: 10.1002/adma.202410452
2024
Viola M, Valverde M, Bernal P, van Trijp J, Hak J, Di Marco G, Neumann M, Schuurmans C, van Nostrum C, Masereeuw R, Mihaila S, Malda J, Levato R, Vermonden T. Advanced Functional Materials. doi: 10.1002/adfm.202406098
Buchholz M, Scheerman D, Levato R, Wehrens E, Rios C. Human breast tissue engineering in health and disease. EMBO Molecular Medicine. doi: 10.1038/s44321-024-00112-3
Meerbothe T, Florczak S, van den Berg C, Levato R, Mandija S. A reusable 3D printed brain-like phantom for benchmarking electrical properties tomography reconstructions. Magnetic Resonance in Medicine. doi: 10.1002/mrm.30189
Bosmans C, Gines-Rodriguez N, Karperien M, Malda J, Texeira-Moreira L, Levato R, Leijten J. Towards single-cell bioprinting: micropatterning tools for organ-on-chip development. Trends in Biotechnology. doi: 10.1016/j.tibtech.2023.11.014
Elkadi O, Abinzano F, Nippolainen E, Bach Gonzalez O, Levato R, Malda J, Afara I. Non-neotissue constituents ad underestimated confounders in the assessment of tissue engineered constructs by near-infrared spectroscopy. Materials Today Bio. doi: 10.1016/j.mtbio.2023.100879
2023
Mancini I, Levato R, Ksiezarcyk M, Castilho M, Chen M, van Rijen M, Ijsseldijk L, Kik M, van Weeren R, Malda J. Microstructural differenes in the osteochondral unit of terrestrial and aquatic mammals. eLife. doi: 10.7554/eLife.80936
de Ruijter M, Diloksumpan P, Dokter I, Brommer H, Smit I, Levato R, van Weeren R, Castilho M, Malda J. Orthotopic equine study confirms the pivotal importance of structural reinforcement over the pre-culture of cartilage implants. Bioengineering & Translational Medicine. doi: 10.1002/btm2.10614
Madrid-Wolff J, Toomb J, Rizzo R, Bernal P, Porcincula D, Walton R, Wang B, Kotz-Helmer F, Yang Y, Kaplan D, Zhang Y, Zenobi-Wong m, McLeod R, Rapp B, Schwartz J, Shusteff M, Taylor H, Levato R, Moser C. A review of materials used in tomographic volumetric additive manufacturing. MRS Communications. doi: 10.1557/s43579-023-00447-x
Tognato R, Parolini R, Jahangir S, Ma J, Florczak S, Richards RG, Levato R, Alini M, Serra T. Sound-based assembly of three-dimensional cellularized and acellularized constructs. Materials Today Bio. doi: 10.1016/j.mtbio.2023.100775
Abbadessa A, Bernal PN, Buttitta G, Ronca A, D'Amora U, Zihlmann C, Stiefel N, Ambrosio L, Malda J, Levato R, Crecente-Campo J, Alonso MJ. Biofunctionalization of 3D printed collagen with bevacizumab-loaded microparticles targeting pathological angiogenesis. Journal of Controlled Release. doi: 10.1016/j.jconrel.2023.07.017
Van Ombergen A, Chalupa-Gantner F, Chansoria P, Colosimo B, Costantini M, Domingos M, Dufour A, De Maria C, Groll J, Jungst T, Levato R, Malda J, Margarita A, Marquette C, Ovsianikov A, Petiot E, Read S, Surdo L, Swieszkowski W, Vozzi G, Windisch J, Zenobi-Wong M, Gelinsky M. 3D Bioprinting in Microgravity: Opportunities, Challenges, and Possible Applications in Space. Advanced Healthcare Materials. doi: 10.1002/adhm.202300443
Levato R, Dudaryeva O, Garciamendez-Mijares C, Kirkpatrick B, Rizzo R, Schimelman J, Anseth K, Chen S, Zenobi-Wong M, Zhang YS. Light-based vat-polymerization bioprinting. Nature Reviews Methods Primers. doi: 10.1038/s43586-023-00231-0
Ribezzi D, Gueye M, Florczak S, Dusi F, de Vos D, Manente F, Hierholzer A, Fussenegger M, Caiazzo M, Blunk T, Malda J, Levato R. Shaping Synthetic Multicellular and Complex Multimaterial Tissues via Embedded Extrusion-Volumetric Printing of Microgels. Advanced Materials. doi: 10.1002/adma.202301673
Galarraga J, Zlotnick H, Locke R, Gupta S, Fogarty N, Masada K, Stoeckl B, Laforest L, Castilho M, Malda J, Levato R, Carey J, Mauck R, Burdick J. Evaluation of Surgical Fixation Methods for the Implantation of Melt Electrowriting-reinforced hyaluronic acid hydrogel composites in porcine cartilage defects. Int J Bioprinting. doi: 10.18063/ijb.775
Falandt M, Bernal P, Dudaryeva O, Florczak S, Größbacher G, Schweiger M, Longoni A, Greant C, Assunção M, Nijssen O, van Vlierberghe S, Malda J, Vermonden T, Levato R. Spatial-Selective Volumetric 4D Printing and Single-Photon Grafting of Biomolecules within Centimeter-Scale Hydrogels via Tomographic Manufacturing. Advanced Materials Technologies. doi: 10.1002/admt.202300026
Größbacher G, Bartolf-Kopp M, Gergely C, Bernal P, Florczak S, de Ruijter M, Gines-Rodriguez N, Groll J, Malda J, Jungst T, Levato R. Volumetric Printing across Melt Electrowritten Scaffolds Fabricates Multi-Material Living Constructs with Tunable Architecture and Mechanics. Advanced Materials. doi: 10.1002/adma.202300756
Rikkers M, Nguyen H, Golafshan N, de Ruijter M, Levato R, Vonk L, van Egmond N, Castilho M, Custers R, Malda J. A Gap-Filling Regenerative Implant for Open-Wedge Osteotomy. Journal of Cartilage & Joint Preservation. doi: 10.1016/j.jcjp.2023.100117
Levato R, Lim K. Harnessing Light in Biofabrication. Biofabrication. doi: 10.1088/1758-5090/acb50f
Soliman B, Longoni A, Wang M, Li W, Bernal P, Cianciosi A, Lindberg G, Malda J, Groll J, Jungst T, Levato R, Rnjak-Kovacina J, Woodfield T, Zhang YS, Lim K. Programming Delayed Dissolution Into Sacrificial Bioinks for Dynamic Temporal Control of Architecture within 3D-Bioprinted Constructs. Advanced Functional Materials. doi: 10.1002/adfm.202210521
2022
Zandrini T, Florczak S, Levato R, Ovsianikov A. Breaking the resolution limits of 3D bioprinting: future opportunities and present challenges. Trends in Biotechnology. doi: 10.1016/j.tibtech.2022.10.009
de Silva L, Bernal P, Rosenberg A, Malda J, Levato R, Gawlitta D. Biofabricating the vascular tree in engineered bone tissue. Acta Biomaterialia. doi: 10.1016/j.actbio.2022.08.051
Otto IA, Bernal P, Rikkers M, van Rijen M, Mensinga A, Kon M, Breugem C, Levato R, Malda J. Human Adult, Pediatric and Microtia Auricular Cartilage harbor Fibronectin-adhering Progenitor Cells with Regenerative Ear Reconstruction Potential. iScience. doi: 10.1016/j.isci.2022.104979
Terpstra M, Li J, Mensinga A, de Ruijter M, van Rijen M, Androulidakis C, Galiotis C, Papantoniou I, Matsusaki M, Malda J, Levato R. Bioink with cartilage-derived extracellular matrix microfibers enables spatial control of vascular capillary formation in bioprinted constructs. Biofabrication. doi: 10.1088/1758-5090/ac6282
Bernal P, Bouwmeester M, Madrid-Wolff J, Falandt M, Florczak S, Gines-Rodriguez N, Grossbacher G, Samsom R, van Wolferen M, van der Laan L, Delrot P, Loterie D, Malda J, Moser C, Spee B, Levato R. Volumetric Bioprinting of Organoids and Optically Tuned Hydrogels to Build Liver-Like Metabolic Biofactories. Advanced Materials. doi: 10.1002/adma.202110054
2021
Longoni A, Utomo L, Robinson A, Rosenberg A, Levato R, Gawlitta D. Acceleration of Bone Regeneration Induced by a Soft-Callus Mimetic Material. Advanced Science. doi: 10.1002/advs.202103284
Levato R, Lim K, Li W, Asua A, Pena L, Wang M, Falandt M, Bernal P, Gawlitta D, Zhang YS, Woodfield T, Malda J. High-resolution lithographic biofabrication of hydrogels with complex microchannels from low-temperature-soluble gelatin bioresins. Materials Today Bio. doi: 10.1016/j.mtbio.2021.100162
Rikkers M, Korpershoek J, Levato R, Malda J, Vonk L. Articular Cartilage-Derived Progenitor Cells in Health and Disease: Potential for Clinical Use? – A Systematic Review of In Vitro and Preclinical Work. npj Regenerative Medicine. doi: 10.1038/s41536-021-00203-6
Rikkers M, Korpershoek J, Levato R, Malda J, Vonk L. Progenitor cells in healthy and osteoarthritic human cartilage have extensive culture expansion capacity while retaining chondrogenic properties. Cartilage. doi: 10.1177/19476035211059600
Bouwemeester M, Bernal P, Oosterhoff L, van Wolferen M, Lehmann V, Vermaas M, Buchholz M, Q. Peiffer Q, Malda J, van der Laan L, Kramer N, Schneeberger K, Levato R, Spee B. Bioprinting of Human Liver-derived Epithelial Organoids for Toxicity Studies. Macromolecular Bioscience. doi: 10.1002/mabi.202100327
Otto I, Capendale P, Garcia J, de Ruijter M, van Droemalen R, Castilho M, Lawson T, Grinstaff M, Breugem C, Kon M, Levato R, Malda J. Biofabrication of a shape-stable auricular structure for the reconstruction of ear deformities. Materials Today Bio. doi: 10.1016/j.mtbio.2021.100094
Castilho M, Levato R, Bernal P, de Ruijter M, Sheng C, van Duijn J, Piluso S, Ito K, Malda J. Hydrogel-based bioinks for cell electrowriting of well-organized living structures with micrometer-scale resolution. Biomacromolecules. doi: 10.1021/acs.biomac.0c01577
Groen W, Utomo L, Castilho M, Gawlitta D, Malda J, van Weeren R, Levato R, Korthagen W. Impact of endotoxins in gelatine hydrogels on chondrogenic differentiation and inflammatory cytokine secretion in vitro. International Journal of Molecular Sciences. doi: 10.3390/ijms21228571
Schmidt S, Abinzano F, Mensinga A, Tessmar J, Groll J, Malda J, Levato R, Blunk T. Differential production of Cartilage ECM in 3D Agarose Constructs by Equine Articular Cartilage Progenitor Cells and Mesenchymal Stromal Cells. International Journal of Molecular Sciences. doi: 10.3390/ijms21197071
Schwab A, Levato R, D’Este M, Piluso S, Eglin D, Malda J. Printability and Shape Fidelity of Bioinks in 3D Bioprinting. Chemical Reviews. doi: 10.1021/acs.chemrev.0c00084
2020
Li J, Sasaki N, Keiji I, Terpstra M, Levato R, Matsusaki M. Regulation of Chondrocyte Differentiation by Changing Intercellular Distances Using Type II Collagen Microfibers. ACS Biomaterials Science & Engineering. doi: 10.1021/acsbiomaterials.0c00427
Rikkers M, Dijkstra K, Terhaard B, Admiraal J, Levato R, Malda J, Vonk L. Platelet-Rich Plasma does not Inhibit Inflammation or Promote Regeneration in Human Osteoarthritic Chondrocytes Despite Increased Proliferation. Cartilage. doi: 10.1177/1947603520961162
Rikkers M, Levato R, Malda J, Vonk L. Importance of timing of platelet lysate-supplementation in expanding or redifferentiating human chondrocytes for chondrogenesis. Frontiers in Bioengineering and Biotechnology. doi: 10.3389/fbioe.2020.00804
Longoni A, Pennings I, Cuenca M, Rijen M, Peperzak V, Levato R Rosenberg A, Gawlitta D. Endochondral bone regeneration by non-autologous mesenchymal stem cells. Frontiers in Bioengineering and Biotechnology. doi: 10.3389/fbioe.2020.00651
Mancini I, Schmidt S, Brommer H, Pouran B, Schafer S, Tessmar J, Mensinga A, van Rijen M, Groll Jm Blunk T, Levato R, Malda J, van Weeren R. A composite hydrogel-3D printed thermoplast osteochondral anchor as an example for a zonal approach to cartilage repair: in vivo performance in a long-term equine model. Biofabrication. doi: 10.1088/1758-5090/ab94ce
Morgan B, Bauza-Mayol G, Gardner O, Zhang Y, Levato R, Archer C, van Weeren R, Malda J, Conlan R, Francis L, Khan I. BMP9 is a potent chondrogenic and morphogenic factor for articular cartilage-derived chondroprogenitors. Stem Cells & Development. doi: 10.1089/scd.2019.0209
Diloksumpan P, Vindas Bolanos R, Cokelaere S, Pouran B, de Grauw J, Rijen M, van Weeren R, Levato R, Malda J. Orthotopic bone regeneration within 3D printed bioceramic scaffolds with region-dependent porosity gradients in an equine model. Advanced Healthcare Materials. doi: 10.1089/scd.2019.0209
Qiu B, Bessler N, Figler K, Buchholz M, Rios A, Malda J, Levato R, Caiazzo M. Bioprinting Neural Systems to Model CNS Diseases. Advanced Functional Materials. 10.1002/adfm.201910250
Lim K, Abinzano F, Bernal P, Albillos-Sancez A, Atienza-Roca P, Otto I, Peiffer Q, Matsusaki M, Woodfield T, Malda J, Levato R. One-step photoactivation of a dual-functionalized bioink as cell carrier and cartilage-binding glue for chondral regeneration. Advanced Healthcare Materials. doi: 10.1002/adhm.201901792
Liu H, Kitano S, Irie S, Levato R, Matsusaki M. Collagen Microfibers Induce Blood Capillary Orientation and Open Vascular Lumen. Advanced Biology. 10.1002/adbi.202000038
Diloksumpan P, de Ruijter M, Castilho M, Gbureck U, Vermonden T, van Weeren R, Malda J, Levato R. Combining multi-scale 3D printing technologies to engineer reinforced hydrogel-ceramic interfaces. Biofabrication. doi: 10.1088/1758-5090/ab69d9
2019
Cui X, Soliman B, Alcala-Orozco C, Li J, Vis M, Santos M, Wise S, Levato R, Malda J, Woodfield T, Rnjack-Kovacina J, Lim K. Rapid Photocrosslinking of Silk Hydrogels with High Cell-Density and Enhanced Shape Fidelity. Advanced Healthcare Materials. doi: 10.1002/adhm.202001801
Augurio A, Cortelletti R, Tognato R, Petta D, Rios A, Levato R, Malda J, Eglin D, Alini M, Giancane G, Speghini A, Serra T. A Multifunctional Nanocomposite Hydrogel for Endoscopic Tracking and Manipulation. Advanced Intelligent Systems. doi: 10.1002/aisy.201900105
Levato R, Jungst T, Scheurer R, Blunk T, Groll J, Malda J. From Shape to Function: the Next Step in Biofabrication. Advanced Materials. doi: 10.1002/adma.201906423
Nuñez-Bernal P, Delrot P, Loterie D, Li Y, Malda J, Moser C, Levato R. Volumetric Bioprinting of Complex Living Tissue Constructs within Seconds. Advanced Materials. doi: 10.1002/adma.201904209
Tognato R, Armiento A, Bonfrate V, Levato R, Malda J, Alini M, Eglin D, Giancane G, Serra T. A Stimuli-Responsive Nanocomposite for 3D Anisotropic Cell-Guidance and Magnetic Soft-Robotics. Advanced Functional Materials. 10.1002/adfm.201804647